PHYCOBILIPROTEINS



Phycobiliproteins

Cyanobacterias as well as the blue and brown seaweed synthesise fluorochrome captors of light called Phycobiliprotein. After purification, these intensive coloured pigments present completely unique spectral properties.
Thanks to its characteristics, these molecules are particularly recommended for fluorescent markings, for immunoessays, for fluorescent microscopy and flow cytometry.


Molecules highly fluorescent in the visible
red-orange spectra (550 to 700 nm)
with a maximum intensity 20 times superior to
fluorescein.
Spectra of fluorescent emission and absorption clearly separated :
Large spectra of excitation separated from the narrow emission reel allows a high sensitive detection and an excellent separation between fluorescent signal and background,

Highly molecular extinction coefficient,
Quantity output is very high (until 98%) for phycoerythrin. They stay constant on a large pH track. This is due to the molecular organisation of the proteic core, thus reducing internal quenching,
High solubility in aqueous solution,
Good stability under solid aspect or in solution,
A low isoelectric point,
Without ose in their molecular structure.


Characteristics :

Phycobiliprotein
Molecular mass

( D )
Excitation

λ max(nm)
Emission

λ max(nm)
Quantique efficiency

( % )
Molecular extinction
coefficient
ε (M-1cm-1)
Allo-phycocyanin
104 000
650
660
68
0.7 106
C-phycocyanin
226 000
620
640
51
1.69 106
B-phycoerythrin
240 000
488,545
575
98
2.41 106

* : Main wavelenghts are underlined.

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